2024.02.18
If you encounter poor cell adhesion, the following steps may help optimize the process:
•Check precoated culture plates and plating medium: Ensure the collagen-precoated culture plates are suitable for cell adhesion. If necessary, use coating solution to prepare your own coated plates to improve the adsorptive quality of the wells; also, select high-quality plating medium appropriate for cell adhesion culture.
•Optimize cell thawing steps: Quickly and effectively revive cells to avoid prolonged operations that lead to decreased cell viability. Strictly follow the correct revival steps to swiftly restore cell vitality.
•Choose appropriate tools: Use pipette tips or Pasteur pipettes of suitable sizes to minimize physical damage to the cells.
•Adjust cell density: Based on the cell type and experimental objectives, adjust the cell density appropriately to avoid adhesion issues caused by too high or too low cell concentrations.
•Prevent contamination: Maintain sterility in the experimental environment, regularly monitor the aseptic status of the culture system to prevent contamination.
