What are the reasons for lower cell counts and how can they ...
Issues with lower cell counts, focusing on post-thawing and during the counting process, mainly involve inaccuracies in counting methods, unsuitable counting chambers, and technical errors.
•First, ensure the cell suspension is thoroughly mixed before counting to avoid undercounting due to sedimentation. Secondly, choosing the appropriate counting chamber is crucial for accuracy; for larger cells like hepatocytes, using a 0.1mm depth counting chamber can reduce counting errors caused by cell overlap or uneven distribution.
•Additionally, if using a manual counting method, it is recommended to count across multiple different fields of view and take the average to reduce random errors. In summary, optimizing the counting method and ensuring operational accuracy can effectively resolve issues with lower cell counts.
